Reliable speciation of fatty acid methyl esters (FAMEs) by flow-modulated GC×GC–TOF MS/FID with Tandem Ionisation

This study demonstrates the use of flow-modulated GC×GC to analyse a FAME standard and FAMEs from a butter extract, with simultaneous detection by TOF MS and FID for improved speciation.

Analysis of FAMEs standard

We show that inverse-phase GC×GC provides the chromatographic resolution necessary for the separation of closely-related isomers in a single analytical run, while Tandem Ionisation adds an additional level of confidence for compounds exhibiting weak molecular ions and/or similar spectra at 70 eV.

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